Structure and expression of nuclear oncogenes in multi-stage thyroid tumorigenesis..pdf (1.34 MB)
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Structure and expression of nuclear oncogenes in multi-stage thyroid tumorigenesis.

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journal contribution
posted on 14.12.2016, 16:42 by F. S. Wyllie, N. R. Lemoine, E. D. Williams, D. Wynford-Thomas
We have investigated the possibility that structural alterations of the 'nuclear' oncogene family (c-myc, N-myc, L-myc, fos, myb and p53) leading to aberrant expression might, as in several other tumour types, play a role in the multi-stage development of tumorigenesis in the human thyroid follicular cell. Direct analysis of expression by slot and Northern blot RNA hybridisation showed that normal thyroid expresses surprisingly high levels of fos, and to a lesser extent c-myc, c-myc expression was markedly increased in all tumours, both benign and malignant, but no increase was seen in any other nuclear oncogene. fos expression was reduced specifically in one type of malignant tumour-follicular carcinoma-in inverse correlation with differentiation. Southern blot analysis showed no evidence of rearrangement or amplification of c-myc, or of any other 'nuclear' oncogene in any thyroid tumour. We conclude that there is no evidence that a primary abnormality of these genes plays a role in thyroid follicular cell tumorigenesis and suggest that the observed changes in expression can be adequately explained as secondary consequences of the tumour phenotype.

Funding

We are grateful to the Cancer Research Campaign of Great Britain for grant support.

History

Citation

British Journal of Cancer (1989) 60, 561–565.

Author affiliation

/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/School of Medicine

Version

VoR (Version of Record)

Published in

British Journal of Cancer (1989) 60

Publisher

Cancer Research UK, Nature Publishing Group

issn

0007-0920

eissn

1532-1827

Available date

14/12/2016

Publisher version

http://www.nature.com/bjc/journal/v60/n4/abs/bjc1989313a.html

Language

en