Telomeric expression sites are highly conserved in Trypanosoma brucei..pdf (1.01 MB)
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Telomeric expression sites are highly conserved in Trypanosoma brucei

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journal contribution
posted on 28.10.2015, 11:29 by C. Hertz-Fowler, L. M. Figueiredo, M. A. Quail, M. Becker, A. Jackson, N. Bason, K. Brooks, C. Churcher, S. Fahkro, I. Goodhead, P. Heath, M. Kartvelishvili, K. Mungall, D. Harris, H. Hauser, M. Sanders, D. Saunders, K. Seeger, S. Sharp, J. E. Taylor, D. Walker, B. White, R. Young, G. A. Cross, G. Rudenko, J. D. Barry, Edward John Louis, M. Berriman
Subtelomeric regions are often under-represented in genome sequences of eukaryotes. One of the best known examples of the use of telomere proximity for adaptive purposes are the bloodstream expression sites (BESs) of the African trypanosome Trypanosoma brucei. To enhance our understanding of BES structure and function in host adaptation and immune evasion, the BES repertoire from the Lister 427 strain of T. brucei were independently tagged and sequenced. BESs are polymorphic in size and structure but reveal a surprisingly conserved architecture in the context of extensive recombination. Very small BESs do exist and many functioning BESs do not contain the full complement of expression site associated genes (ESAGs). The consequences of duplicated or missing ESAGs, including ESAG9, a newly named ESAG12, and additional variant surface glycoprotein genes (VSGs) were evaluated by functional assays after BESs were tagged with a drug-resistance gene. Phylogenetic analysis of constituent ESAG families suggests that BESs are sequence mosaics and that extensive recombination has shaped the evolution of the BES repertoire. This work opens important perspectives in understanding the molecular mechanisms of antigenic variation, a widely used strategy for immune evasion in pathogens, and telomere biology.

History

Citation

PLoS One, 2008, 3 (10), e3527

Author affiliation

/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/MBSP Non-Medical Departments/Department of Genetics

Version

VoR (Version of Record)

Published in

PLoS One

Publisher

Public Library of Science

eissn

1932-6203

Acceptance date

27/09/2008

Copyright date

2008

Available date

28/10/2015

Publisher version

http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0003527

Language

en