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Temperature dependent bacteriophages of a tropical bacterial pathogen

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journal contribution
posted on 06.03.2015, 16:40 by Jinyu Shan, S. Korbsrisate, P. Withatanung, Natalie Lazar Adler, Martha R. J. Clokie, Edouard E. Galyov
There is an increasing awareness of the multiple ways that bacteriophages (phages) influence bacterial evolution, population dynamics, physiology, and pathogenicity. By studying a novel group of phages infecting a soil borne pathogen, we revealed a paradigm shifting observation that the phages switch their lifestyle according to temperature. We sampled soil from an endemic area of the serious tropical pathogen Burkholderia pseudomallei, and established that podoviruses infecting the pathogen are frequently present in soil, and many of them are naturally occurring variants of a common virus type. Experiments on one phage in the related model B. thailandensis demonstrated that temperature defines the outcome of phage-bacteria interactions. At higher temperatures (37◦C), the phage predominantly goes through a lytic cycle, but at lower temperatures (25◦C), the phage remains temperate. This is the first report of a naturally occurring phage that follows a lytic or temperate lifestyle according to temperature.These observations fundamentally alter the accepted views on the abundance, population biology and virulence of B. pseudomallei. Furthermore, when taken together with previous studies, our findings suggest that the phenomenon of temperature dependency in phages is widespread. Such phages are likely to have a profound effect on bacterial biology, and on our ability to culture and correctly enumerate viable bacteria.

Funding

This work was supported by the Wellcome Trust, UK (Grant number 092638/Z/10/Z).

History

Citation

Frontiers in Microbiology, 2014, 5:599

Author affiliation

/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/School of Medicine/Department of Infection, Immunity and Inflammation

Version

VoR (Version of Record)

Published in

Frontiers in Microbiology

issn

1664-302X

eissn

1664-302X

Copyright date

2014

Available date

06/03/2015

Publisher version

http://journal.frontiersin.org/article/10.3389/fmicb.2014.00599/abstract

Language

en