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The IMPROVE Guidelines (Ischaemia Models: Procedural Refinements Of in Vivo Experiments)

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journal contribution
posted on 01.05.2018, 09:04 by N. Percie du Sert, A. Alfieri, S. M. Allan, H. V. Carswell, G. A. Deuchar, T. D. Farr, P. Flecknell, L. Gallagher, Claire L. Gibson, M. J. Haley, M. R. Macleod, B. W. McColl, C. McCabe, A. Morancho, L. D. Moon, M. J. O'Neill, I. Pérez de Puig, A. Planas, C. I. Ragan, A. Rosell, L. A. Roy, K. O. Ryder, A. Simats, E. S. Sena, B. A. Sutherland, M. D. Tricklebank, R. C. Trueman, L. Whitfield, R. Wong, I. M. Macrae
Most in vivo models of ischaemic stroke target the middle cerebral artery and a spectrum of stroke severities, from mild to substantial, can be achieved. This review describes opportunities to improve the in vivo modelling of ischaemic stroke and animal welfare. It provides a number of recommendations to minimise the level of severity in the most common rodent models of middle cerebral artery occlusion, while sustaining or improving the scientific outcomes. The recommendations cover basic requirements pre-surgery, selecting the most appropriate anaesthetic and analgesic regimen, as well as intraoperative and post-operative care. The aim is to provide support for researchers and animal care staff to refine their procedures and practices, and implement small incremental changes to improve the welfare of the animals used and to answer the scientific question under investigation. All recommendations are recapitulated in a summary poster (see supplementary information).

History

Citation

Journal of Cerebral Blood Flow and Metabolism, 2017, 37 (11), pp. 3488-3517

Author affiliation

/Organisation/COLLEGE OF LIFE SCIENCES/Biological Sciences/Neuroscience, Psychology and Behaviour

Version

VoR (Version of Record)

Published in

Journal of Cerebral Blood Flow and Metabolism

Publisher

SAGE Publications

issn

0271-678X

eissn

1559-7016

Acceptance date

13/03/2017

Copyright date

2017

Available date

01/05/2018

Publisher version

http://journals.sagepub.com/doi/10.1177/0271678X17709185

Language

en