Tissue specific expression of p73 C terminal isoforms in mice.pdf (1.42 MB)
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Tissue-specific expression of p73 C-terminal isoforms in mice

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journal contribution
posted on 26.02.2016, 10:36 by Francesca Grespi, Ivano Amelio, Paola Tucci, M. Annicchiarico-Petruzzelli, Gerry Melino
p73 is a p53 family transcription factor. Due to the presence in the 5′ flanking region of two promoters, there are two N-terminal variants, TAp73, which retains a fully active transactivation domain (TA), and ΔNp73, in which the N terminus is truncated. In addition, extensive 3′ splicing gives rise to at least seven distinctive isoforms; TAp73-selective knockout highlights its role as a regulator of cell death, senescence and tumor suppressor. ΔNp73-selective knockout, on the other hand, highlights anti-apoptotic function of ΔNp73 and its involvement in DNA damage response. In this work, we investigated the expression pattern of murine p73 C-terminal isoforms. By using a RT-PCR approach, we were able to detect mRNAs of all the C-terminal isoforms described in humans. We characterized their in vivo expression profile in mouse organs and in different mouse developmental stages. Finally, we investigated p73 C-terminal expression profile following DNA damage, ex vivo after primary cultures treatment and in vivo after systemic administration of cytotoxic compounds. Overall, our study first elucidates spatio-temporal expression of mouse p73 isoforms and provides novel insights on their expression-switch under triggered conditions.

Funding

This work has been supported by the Medical Research Council, United Kingdom; MIUR, MinSan, RF73, RF57, ACC12; Odysseus Grant (G.0017.12) from the Flemish government and Flanders Institute for Biotechnology, Belgium.

History

Citation

Cell Cycle 11:23, 4474–4483; December 1, 2012;

Version

VoR (Version of Record)

Published in

Cell Cycle 11:23

Publisher

Landes Bioscience, Taylor & Francis

issn

1538-4101

eissn

1551-4005

Copyright date

2012

Available date

26/02/2016

Publisher version

http://www.tandfonline.com/doi/abs/10.4161/cc.22787

Notes

Supplemental materials may be found here: www.landesbioscience.com/admin/article/22787/

Language

en

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