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Transmission distortion affecting human noncrossover but not crossover recombination : a hidden source of meiotic drive

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posted on 20.03.2014, 09:25 by Linda Odenthal-Hesse, Ingrid L. Berg, Amelia Veselis, Alec J. Jeffreys, Celia A. May
Meiotic recombination ensures the correct segregation of homologous chromosomes during gamete formation and contributes to DNA diversity through both large-scale reciprocal crossovers and very localised gene conversion events, also known as noncrossovers. Considerable progress has been made in understanding factors such as PRDM9 and SNP variants that influence the initiation of recombination at human hotspots but very little is known about factors acting downstream. To address this, we simultaneously analysed both types of recombinant molecule in sperm DNA at six highly active hotspots, and looked for disparity in the transmission of allelic variants indicative of any cis-acting influences. At two of the hotspots we identified a novel form of biased transmission that was exclusive to the noncrossover class of recombinant, and which presumably arises through differences between crossovers and noncrossovers in heteroduplex formation and biased mismatch repair. This form of biased gene conversion is not predicted to influence hotspot activity as previously noted for SNPs that affect recombination initiation, but does constitute a powerful and previously undetected source of recombination-driven meiotic drive that by extrapolation may affect thousands of recombination hotspots throughout the human genome. Intriguingly, at both of the hotspots described here, this drive favours strong (G/C) over weak (A/T) base pairs as might be predicted from the well-established correlations between high GC content and recombination activity in mammalian genomes.

Funding

This work was supported by grants from the Medical Research Council (to AJJ and CAM), Louis-Jeantet Foundation (to AJJ) and Royal Society (to AJJ). LOH was supported by Boehringer Ingelheim Fonds.

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Citation

PLoS Genetics, 2014, 10 (2), e1004106

Author affiliation

/Organisation/COLLEGE OF MEDICINE, BIOLOGICAL SCIENCES AND PSYCHOLOGY/School of Biological Sciences/Department of Genetics

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VoR (Version of Record)

Published in

PLoS Genetics

Publisher

Public Library of Science

issn

1553-7390

eissn

1553-7404

Copyright date

2014

Available date

20/03/2014

Publisher version

http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004106

Language

en

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