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ZYP1 is required for obligate cross-over formation and cross-over interference in Arabidopsis

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posted on 15.11.2021, 17:26 by Martin G France, Janina Enderle, Sarah Röhrig, Holger Puchta, F Chris H Franklin, James D Higgins
The synaptonemal complex is a tripartite proteinaceous ultrastructure that forms between homologous chromosomes during prophase I of meiosis in the majority of eukaryotes. It is characterized by the coordinated installation of transverse filament proteins between two lateral elements and is required for wild-type levels of crossing over and meiotic progression. We have generated null mutants of the duplicatedArabidopsistransverse filament geneszyp1aandzyp1busing a combination of T-DNA insertional mutants and targeted CRISPR/Cas mutagenesis. Cytological and genetic analysis of thezyp1null mutants reveals loss of the obligate chiasma, an increase in recombination map length by 1.3- to 1.7-fold and a virtual absence of cross-over (CO) interference, determined by a significant increase in the number of double COs. At diplotene, the numbers of HEI10 foci, a marker for Class I interference-sensitive COs, are twofold greater in thezyp1mutant compared to wild type. The increase in recombination inzyp1does not appear to be due to the Class II interference-insensitive COs as chiasmata were reduced by ∼52% inmsh5/zyp1compared tomsh5. These data suggest that ZYP1 limits the formation of closely spaced Class I COs inArabidopsis. Our data indicate that installation of ZYP1 occurs at ASY1-labeled axial bridges and that loss of the protein disrupts progressive coalignment of the chromosome axes.

Funding

United Kingdom Research and Innovation through Biological and Biotechnological Sciences Research Council Midlands Integrative Biosciences Training Partnership (BB/M01116X/1)

History

Citation

PNAS April 6, 2021 118 (14) e2021671118; https://doi.org/10.1073/pnas.2021671118

Author affiliation

Department of Genetics and Genome Biology, University of Leicester

Version

VoR (Version of Record)

Published in

Proceedings of the National Academy of Sciences

Volume

118

Issue

14

Pagination

e2021671118 - e2021671118

Publisher

Proceedings of the National Academy of Sciences

issn

0027-8424

eissn

1091-6490

Copyright date

2021

Available date

15/11/2021

Language

en

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