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halfman, an Arabidopsis male gametophytic mutant associated with a 150 kb chromosomal deletion at the site of transposon insertion

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posted on 01.09.2006, 15:31 by Sung-Aeong Oh, Soon Ki Park, Inok Jang, Ross Howden, James M. Moore, Ueli Grossniklaus, David Twell
To identify genes that play an important gametophytic role during pollen development, an Arabidopsis transposon (DsE) mutagenised population was screened for marker segregation ratio distortion. We report the characterisation of a male gametophytic mutant termed halfman (ham) that results in an aborted pollen phenotype in mature anthers. The genetic transmission efficiency of DsE was 6.6% through the male and 98.4% through the female, which suggested that HAM may encode essential male-specific component(s) required for pollen development. Molecular analysis of the insertion site revealed a single copy of the DsE element inserted into the second exon of the RLK5 gene that was adjacent to a large (~150 Kb) genomic deletion. The deleted region is predicted to encode 38 genes and to include one or more genes with important function(s) during pollen maturation and seed development.

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Citation

Sexual Plant Reproduction, 2003, 16, pp. 99-102

Published in

Sexual Plant Reproduction

Publisher

Springer Verlag

Available date

01/09/2006

Publisher version

http://link.springer.com/article/10.1007/s00497-003-0181-9

Notes

This is the authors' final version of a paper published as Sexual Plant Reproduction, 2003, 16, pp.99-102. The original publication is available at www.springerlink.com

Language

en

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