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Structural, functional and mechanistic analysis of the Bag-1 internal ribosome entry site

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posted on 15.12.2014, 10:32 by Becky M. Pickering
Bag-1 is an anti-apoptotic protein involved in the regulation of a number of cellular processes, notably as a co-chaperone for the 70kDa heat shock proteins. At least four protein products of Bag-1 have been isolated, p50, p46, p36 and a minor isoform, p29. The 5' UTR of the p36 isoform of Bag-1 has been shown to contain an internal ribosome entry segment (IRES). The internal ribosome entry mediated mechanism of translation has been shown to maintain Bag-1 expression when cap- dependent translation is compromised during heat shock. Many IRESes require trans-acting protein factors for optimal IRES activity. Bag-1 IRES activity is cell-type specific and is inefficient in cell lines with low endogenous levels of the /r

History

Date of award

01/01/2003

Author affiliation

Biochemistry

Awarding institution

University of Leicester

Qualification level

Doctoral

Qualification name

PhD

Language

en

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